کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4557847 | 1329901 | 2013 | 8 صفحه PDF | دانلود رایگان |

• The first transcriptomic investigation in mantle tissue from manila clams during in vivo V. tapetis infection.
• Contribution of transcripts involved in immunity and biomineralization during BRD defense processes.
• Two specific genes identified in mantle tissue were not expressed in hemocytes during infection.
Severe drop in Manila clams production in French aquacultured fields since the end of the 1980’s is associated to Brown Ring Disease (BRD). This disease, caused by the bacteria Vibrio tapetis, is characterized by specific symptoms on the inner face of the shell. Diseased animals develop conchiolin deposit to enrobe bacteria and form new calcified layers on the shell. Suppression subtractive hybridization was performed to identify genes differentially expressed during the early interaction of V. tapetis and Ruditapes philippinarum. Results revealed 301 unique genes differentially expressed during V. tapetis challenge. Several candidates involved in immune and biomineralization processes were selected from libraries. Transcriptional expression of selected candidates was determined in hemolymph and mantle tissues and revealed spatial and temporal variations. At 56 days after infection, when clams were in phase of shell repair, transcripts of galectin and ferritin in hemocytes showed higher expression. Ca-like and serpin transcripts were specifically expressed in mantle and could contribute to defense against BRD.
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Journal: Journal of Invertebrate Pathology - Volume 113, Issue 2, June 2013, Pages 129–136