کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4558833 1329964 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of methods for estimating the prevalence of Neozygites floridana in Tetranychus urticae populations infesting strawberries
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Comparison of methods for estimating the prevalence of Neozygites floridana in Tetranychus urticae populations infesting strawberries
چکیده انگلیسی

Methods for measuring prevalence of Neozygites floridana in a Tetranychus urticae population collected from strawberries were developed and compared. T. urticae were extracted from leaves using a soapy water solution (0.5 ml washing detergent : 8 L water) and then placed into 80% alcohol for use in Methods 1 and 2. Method 1: N. floridana-sporulating T. urticae cadavers were observed and quantified under a compound microscope (40–80×). Method 2: Adult females were mounted in lactophenol cotton blue and observed for the presence or absence of N. floridana hyphal bodies under a microscope (200–400×). Method 3: Live T. urticae females were incubated at 25 °C and 75% RH and observed for mortality and N. floridana infection under a compound microscope (6.4–40×). Method 1 was the most time-efficient method and it also allows processing of samples as time permits. Method 2 quantified significantly higher fungal prevalence than Methods 1 and 3, but Method 2 is not considered to be reliable because hyphal bodies are difficult to detect. No significant differences were found between Methods 1 and 3.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Invertebrate Pathology - Volume 92, Issue 1, May 2006, Pages 1–6
نویسندگان
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