A first genetic map of Acca sellowiana based on ISSR, AFLP and SSR markers

• We constructed the first integrated linkage map of Acca sellowiana (2n = 2x = 22).
• For map construction, a multipoint approach that also provides a simultaneous estimation of linkage and linkage phases based on maximum-likelihood analysis was followed.
• The integrated map (2875 cM) included 219 markers distributed in 10 major linkage groups with 91% of genome coverage.
• Three of the major LG combined both TCO (female) and BR (male)-specific LG.
• This study is the foundation for future QTL mapping, molecular breeding and genomic studies of the species.

A first integrated linkage map of Acca sellowiana (Berg.) Burret (2n = 2x = 22), commonly known as ‘pineapple guava’ was constructed using a 160 (TCO × BR) full-sib F1 progeny based on intersimple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. To develop an integrated map, we used a novel statistical approach that provided a simultaneous estimation of linkage and linkage phases based on maximum-likelihood analysis. The integrated map comprised 219 (27 ISSRs and 192 AFLPs) linked markers that were assigned to 10 major linkage groups (LG) representing a total map length of 2875 cM and average distance between markers of 13.8 cM. TCO (14 LG, 83 markers, 1264.6 cM) and BR (18 LG, 94 markers, 1295.1 cM) parent maps were also constructed through the same statistical approach but using only appropriate pseudo-testcross configuration markers. All the LG of the integrated map shared markers with either TCO or, BR or both parental maps. The integrated map provides a framework for further genetic and genomic analyses in A. sellowiana.