کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4566874 1628829 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High frequency somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of gherkin (Cucumis anguria L.)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
High frequency somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of gherkin (Cucumis anguria L.)
چکیده انگلیسی


• Direct SE was induced from the hypocotyl and leaf explants of gherkin.
• The sucrose (5%), 2,4-D, BAP and l-glutamine to induce somatic embryogenesis.
• Activated charcoal (AC) had a positive effect on embryo development.
• Plantlet formation of somatic embryos was positively influenced by AC and GA3.

Gherkin (Cucumis anguria L.) is an important commercial vegetable crop. An efficient protocol for plantlet regeneration from hypocotyl and leaf explants through direct somatic embryogenesis was developed. High frequency of somatic embryos (21.6 and 34.0) were obtained from hypocotyl and leaf explants when cultured on Murashige and Skoog (MS) salts plus B5 vitamins (MSB5) supplemented with 5% sucrose, 1.5 mg L−1 2,4-D, 0.5 mg L−1 BAP and 150 μM l-glutamine. Initial culturing of embryos in the dark conditions for two weeks, followed by four weeks under light resulted in a higher frequency of embryo formation when compared to continuous light conditions. Histological observation showed that the somatic embryos originated from single cells of the epidermal layer. Histological evidence on formation of shoot and root poles during conversion of the embryos confirmed that these structures were true somatic embryos. Maturation of somatic embryos occurred on plant growth regulator-free MSB5 semi-solid medium containing 3% sucrose and 0.5% (w/v) activated charcoal (AC). Conversion of embryos into plantlets was achieved on MSB5 medium supplemented with 3% sucrose, 0.3% AC and 0.5 mg L−1 gibberellic acid (GA3). Sucrose was found to be the best carbon source for SE induction, maturation and germination. Ninety percent of embryos were converted into normal plantlets. The plantlets were successfully acclimatized in the greenhouse with 95% survival rate and transferred to ex vitro conditions which developed with normal phenotypes. This regeneration protocol assured successful embryo induction and plantlet conversion. This is the first report for the induction of direct somatic embryogenesis from hypocotyl and leaf explants of C. anguria. The result of this study is beneficial for genetic transformation and mass clonal propagation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 169, 16 April 2014, Pages 161–168
نویسندگان
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