کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4566953 1628838 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Establishment of photomixotrophic cultures for raspberry micropropagation in Temporary Immersion Bioreactors (TIBs)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
Establishment of photomixotrophic cultures for raspberry micropropagation in Temporary Immersion Bioreactors (TIBs)
چکیده انگلیسی

A novel protocol for raspberry (Rubus spp.) micropropagation in photomixotrophic conditions was optimized for the commercial genotypes Heritage, Meeker and Amity. Plant cultures were established in Temporary Immersion Bioreactors (TIBs) under a controlled environment: 550 ppmv CO2; light intensity 80 μM m−2 s−1; sucrose concentrations 15 gr/L and 30 gr/L. Results showed that both CO2 flux and chlorophyll fluorescence were increased in plants cultured in TIBs with sucrose-reduced medium (15 gr/L) in comparison with those plants micropropagated in TIBs + 30 gr/L sucrose, confirming a consistent photomixotrophic stage since the 5th day of culture. Raspberry plants multiplied in TIBs + 15 gr/L sucrose demonstrated the highest values of plant size, total number of internodes, and percent of acclimatization to greenhouse conditions. Studied variables could be related to an increase of the in vitro photosynthetic activity which might prime plants for the ex vitro environment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 160, 27 August 2013, Pages 49–53
نویسندگان
, , , , ,