کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4567316 1628842 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genetic linkage maps of centipedegrass [Eremochloa ophiuroides (Munro) Hack] based on sequence-related amplified polymorphism and expressed sequence tag-simple sequence repeat markers
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
Genetic linkage maps of centipedegrass [Eremochloa ophiuroides (Munro) Hack] based on sequence-related amplified polymorphism and expressed sequence tag-simple sequence repeat markers
چکیده انگلیسی


• We construct the first genetic maps of centipedegrass using PCR-based markers.
• Two genetic maps are constructed by double pseudo-testcross mapping strategy.
• A combination of SRAP and EST-SSRs from wheat is used for map construction.
• The female maps cover 623.6 cM and the male maps cover 673.1 cM.
• This study establishes a foundation for future molecular breeding in this species.

Centipedegrass [Eremochloa ophiuroides (Munro) Hack] is a warm-season turfgrass native to the southeast of China. In this study, the first genetic linkage maps of centipedegrass were constructed using PCR-based markers based on a segregation population consisting of 89 F1 progeny derived from a cross between two ecotypes (E102 and E092(1)). A combination of sequence-related amplified polymorphisms (SRAPs) and expressed sequence tag-simple sequence repeats (EST-SSRs) from wheat was used for map construction. Eighty nine loci, including 85 SRAP and 4 EST-SSR loci, in the female (E102) linkage map were placed in five major and five minor (two triplet and three doublets) linkage maps, covering 623.6 cM with an average map distance of 7.1 cM between adjacent markers. In the male (E092(1)) linkage map, 71 loci including 71 SRAP and 0 EST-SSR loci were assigned to seven major and five triplets linkage maps, with genome coverage of 673.1 cM, and a mean inter-marker separation of 9.6 cM. The information presented in this study establishes a foundation for extending genetic mapping in this species, serves as a framework for mapping quantitative trait loci, and provides basic information for future molecular breeding.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 156, 7 June 2013, Pages 86–92
نویسندگان
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