Isolation, characterization and expression analysis of the genes-GhAOS, GhAOC and GhOPR3: Encoding the key enzymes involved in jasmonic acid biosynthesis in Gladiolus hybridus

► We isolated the GhAOS, GhAOC and GhOPR3 genes in full length from in vitro developing corms of Gladiolus hybridus. ► We analyzed the three genes expression patterns in different organs of G. hybridus. ► We observed that MJ treatments contributed to increasing the amount of three genes, mRNA level and endogenous MJ content. ► The three genes expression contributed to JA biosynthesis thus playing an important role in corm formation and enlargement.