کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4567877 | 1628868 | 2011 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Molecular cloning and characterization of a novel gene encoding an EF-hand calcium-binding protein related to fruit seedlessness of grapevine Molecular cloning and characterization of a novel gene encoding an EF-hand calcium-binding protein related to fruit seedlessness of grapevine](/preview/png/4567877.png)
EF-hand calcium-binding protein (CBP) gene plays a critical role during embryonic development in animals, but it remains unclear whether this gene possesses similar function in plants. In this study, a novel CBP gene was isolated from Vitis vinifera cv. Thompson Seedless and designated as VvCBP1. Using a combination of sequence structural investigation, expression pattern analysis of different embryo development in grapevine, and silencing assay of VvCBP1 homolog in tomato, the functional role of VvCBP gene in embryo development of seedless grapevine was studied. Structural investigations showed that VvCBP1 is 907 bp in length, and contains two EF-hand motifs. Expression pattern of this gene is different in embryo between seedless and seeded grapevine species. Moreover, the silencing of homology gene led to the seeds number significantly decreased in tomato. These data demonstrate clearly that VvCBP1 plays an important role in embryo development of seedless grapevine.
► We isolated a novel EF-hand calcium-binding protein (CBP) gene from seedless grapevine.
► We examined expression changes of the CBP gene at different embryo development.
► Silencing of homology gene in tomato led to the seeds number significantly decreased.
► The gene may be associated with embryo development of seedlessness in grapevine.
Journal: Scientia Horticulturae - Volume 130, Issue 4, 31 October 2011, Pages 708–714