کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4568246 1331292 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of transgenic sweetpotato plants resistant to stem nematodes using oryzacystatin-I gene
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
Production of transgenic sweetpotato plants resistant to stem nematodes using oryzacystatin-I gene
چکیده انگلیسی

Stem nematode (Ditylenchus destructor Thorne) is one of most serious diseases limiting sweetpotato (Ipomoea batatas (L.) Lam.) production, and it is urgent to develop sweetpotato varieties resistant to this disease. In present study, we have developed transgenic sweetpotato (cv. Xushu 18) plants resistant to stem nematodes using oryzacystatin-I (OCI) gene with Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105 harbors a binary vector pCAMBIA1301 with OCI gene, uidA gene and hptII gene. Selection culture was conducted using 7 mg/l hygromycin. A total of 2119 plants were produced from the inoculated 1710 cell aggregates of Xushu 18 via somatic embryogenesis. GUS assay and PCR analysis of the regenerated plants randomly sampled showed that 92.8% of the regenerated plants were transgenic plants. Transgenic plants exhibited enhanced resistance to stem nematodes compared to the untransformed control plants by the field evaluation and the inoculation test with stem nematodes and stem nematode-resistant plants were selected from the transgenic plants. Stable integration of the OCI gene into the genome of resistant transgenic plants was confirmed by Southern blot analysis, and the copy number of integrated OCI gene ranged from 1 to 3. High level of OCI gene expression in the resistant transgenic plants was demonstrated by real-time quantitative PCR analysis. This study provides a way for improving stem nematode resistance of sweetpotato.


► OCI gene enhanced stem nematode resistance of sweetpotato.
► Stem nematode-resistant transgenic sweetpotato was produced.
► Cell aggregates were efficient receptor for sweetpotato transformation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 128, Issue 4, 10 May 2011, Pages 408–414
نویسندگان
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