کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4568472 | 1628869 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ectopic expression of serotonin N-hydroxycinnamoyltransferase and differential production of phenylpropanoid amides in transgenic tomato tissues
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Tomato contains high levels of amines such as serotonin and tyramine and is a suitable host to enhance phenylpropanoid amides (PAs), an important class of nutraceuticals with strong antioxidant activity and chemotherapeutic effects, by ectopic expression of the corresponding gene, serotonin N-hydroxycinnamoyltransferase (SHT). To assess whether ectopic overexpression of SHT cDNA under the control of the CaMV 35S promoter would enhance levels of PAs, we generated transgenic tomato plants and analyzed the levels of PAs. Transgenic tomato plants exhibited increased synthesis of PAs such as feruloylserotonin (FS), 4-coumaroylserotonin (CS), feruloyltyramine (FT), 4-coumaroyltyramine (CT), and feruloyloctopamine (FO) in 1-month-old leaves compared to the wild type. The increase and relative levels of PAs were even more apparent in 3-month-old leaves of transgenic tomato. When tomato leaves were challenged by wounding, levels of PAs in the best transgenic line increased by 3- and 10-fold for CSÂ +Â FS and CTÂ +Â FT, respectively. In contrast to leaves, tomato fruit only showed enhanced synthesis of CTÂ +Â FT, whereas CSÂ +Â FS levels were not enhanced. Regarding amine content, the levels of tyramine were much higher than those of serotonin in tomato leaves and fruits. The high levels of tyramine may contribute to the preferential production of CTÂ +Â FT rather than CSÂ +Â FS, although SHT enzyme shows the highest substrate affinity toward serotonin rather than tyramine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 120, Issue 4, 19 May 2009, Pages 504-510
Journal: Scientia Horticulturae - Volume 120, Issue 4, 19 May 2009, Pages 504-510
نویسندگان
Kiyoon Kang, Kyungjin Lee, Sung-Oh Sohn, Sangkyu Park, Sungbeom Lee, Soo Young Kim, Young Soon Kim, Kyoungwhan Back,