کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4568774 | 1331312 | 2010 | 7 صفحه PDF | دانلود رایگان |
Chalcone synthase (CHS) and chalcone isomerase (CHI) are two key genes involved in flavonoid biosynthesis. They were both cloned from Guoqing No. 4 satsuma mandarin (Citrus unshiu Marcow), and their relative expression and accumulation of corresponding flavonoid components during fruit maturation were investigated by real-time PCR and HPLC techniques, respectively. During fruit maturation, expression of CHS and CHI genes declined gradually in peels, as well as the concentrations of total flavonoids, trans-chalcone, narirutin and hesperidin; in pulps, however, expression of both genes showed an approximately uptrend, and the concentrations of total flavonoids and those three components were detected in a lower level without significant changes among different developmental periods (P < 0.05). This research confirmed that expression of CHS and CHI genes was positively correlated with flavonoid accumulation and overexpression of them could be a potential approach to produce massive desired flavonoids in citrus fruits.
Journal: Scientia Horticulturae - Volume 125, Issue 2, 3 June 2010, Pages 110–116