کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4568881 1331315 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A practical and reliable procedure for in vitro induction of tetraploid tomato
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
A practical and reliable procedure for in vitro induction of tetraploid tomato
چکیده انگلیسی

A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 122, Issue 3, 1 October 2009, Pages 501–505
نویسندگان
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