Molecular analysis of introgression lines from Cucumis hystrix Chakr. to C. sativus L

Two stable introgression lines (ILs), IL56 and IL73 with fourteen chromosomes, derived from a cross between the wild relative Cucumis hystrix Chakr. (2n = 24) and the cultivated cucumber “beijingjietou” (Cucumis sativus L., 2n = 14) were characterized using SSR and AFLP markers. Twenty-three SSR primers were used to detect the introgression from C. hystrix to C. sativus, and one locus at 210 bp was revealed and assigned to introgressive fragment of C. hystrix genome in IL56. Substantial genomic changes in these two lines were detected by AFLP analysis with thirty different primer combinations. C. hystrix-specific bands, novel bands for the two parents and deleted bands in C. sativus were observed. One hundred and forty-seven polymorphic bands were observed, but only four and five introgressed bands were found in IL56 and IL73, respectively. Although representing only a small proportion of the C. hystrix genome, these two introgression lines with downy mildew and fusarium wilt resistance will be valuable to resistant genetics and breeding.