کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4569173 1331326 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower)
چکیده انگلیسی

Adventitious shoot regeneration and protoplast isolation and culture were examined from leaf explants of in vitro shoot cultures of several cauliflower (Brassica oleracea var. botrytis) cultivars, sourced from Europe and Australia, was investigated with the aim to develop improved nuclear and plastid transformation protocols for this vegetable crop. Eight out of 10 cultivars regenerated shoots from at least 79% of leaf explants. Mesophyll protoplasts from leaves gave high yields and division frequencies. Growth of shoot cultures in large glass vessels with vented lids was the key factor in obtaining high protoplast division frequencies of up to 71% and at least 70% of protoplast calluses regenerating shoots.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 119, Issue 3, 3 February 2009, Pages 330–334
نویسندگان
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