کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4569294 | 1331331 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fruit yield and quality of strawberry plants transformed with a fruit specific strawberry pectate lyase gene
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Fruit yield and quality of strawberry plants transformed with a fruit specific strawberry pectate lyase gene Fruit yield and quality of strawberry plants transformed with a fruit specific strawberry pectate lyase gene](/preview/png/4569294.png)
چکیده انگلیسی
Two transgenic strawberry lines (Pel 1 and Pel 3) containing the open reading frame of a fruit specific strawberry pectate lyase gene (FaplC) under the control of the CaMV35S promoter have been obtained to evaluate the role of this gene on fruit softening. Ripen fruits from both lines showed a significant down-regulation of FaplC, being the percentage of silencing of 84 and 71% on Pel 1 and Pel 3, respectively. The agronomic behaviour of transgenic plants was evaluated during two consecutive years. Fruit set increased in the two transgenic lines when compared with control plants, although Pel 1 showed a significant reduction on fruit weight. Firmness of full ripen fruits from Pel lines was significantly higher than control fruits, while color and soluble solids were not affected. The increase of firmness in Pel lines was maintained when ripe fruits were stored for 3 days at 25 °C. Histological analysis of ripe fruits showed lower intercellular spaces and a higher degree of cell to cell contact area in transgenic fruits when compared with controls. Altogether, these results suggest a direct relationship between pectate lyase gene expression and strawberry fruit softening.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 119, Issue 2, 6 January 2009, Pages 120-125
Journal: Scientia Horticulturae - Volume 119, Issue 2, 6 January 2009, Pages 120-125
نویسندگان
Sabry M. Youssef, Silvia Jiménez-Bermúdez, M. Luz Bellido, Carmen MartÃn-Pizarro, Marta Barceló, Samia Abdallah Abdal-Aziz, José L. Caballero, José M. López-Aranda, Fernando Pliego-Alfaro, Juan Muñoz, Miguel A. Quesada, José A. Mercado,