کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4569482 1331340 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Encapsulation of shoot tips of guava (Psidium guajava L.) for short-term storage and germplasm exchange
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
Encapsulation of shoot tips of guava (Psidium guajava L.) for short-term storage and germplasm exchange
چکیده انگلیسی

Shoot tips obtained from in vitro grown plantlets of guava (Psidium guajava L.) were encapsulated in calcium alginate beads for short-term storage and germplasm exchange. A gelling matrix of 3% sodium alginate and 100 mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Maximum percent response for conversion of encapsulated shoot tips into plantlets was obtained on growth regulator free full strength liquid MS medium. The regrowth ability of encapsulated shoot tips was affected by medium strength and sucrose concentrations in the medium. Encapsulated shoot tips could be stored at low temperature (4 °C) up to 30 days with a survival frequency of 25%. After 60 days of storage under minimal growth conditions (sucrose lacking medium), about 75% encapsulated shoot tips were converted into plantlets when subcultured on 3% sucrose containing medium. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 118, Issue 1, 2 September 2008, Pages 33–38
نویسندگان
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