کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4569685 | 1331350 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of differentially expressed genes in fragrant rose Jinyindao with suppressive subtraction hybridization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To investigate the floral fragrance new genes, scent mutant of rose was used here. The suppressive subtraction hybridization (SSH) technique and micoarray analysis of the clones were used to isolate the cDNA fragments, which showed differential expression between the rose scent mutant 'Wangriqinghuai' and wild type 'Jinyindao' (Rosa Ã hybrida), and RT-PCR was used to identify up-regulated expressed genes. 16 positive contigs of JSSH were obtained. Some ESTs such as RcOMT1, RcOMT2, RhMYB92 and RhGP1 were known to regulate scent metabolism, and 5 ESTs with no homology in NCBI may represent new genes involved in rose flower fragrance metabolism. SSH technique combined with cDNA micoarray would be useful for analysis and isolation of the genes related to rose floral scent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 116, Issue 3, 1 May 2008, Pages 318-323
Journal: Scientia Horticulturae - Volume 116, Issue 3, 1 May 2008, Pages 318-323
نویسندگان
Xie Jirong, Xiong Yunhai, Liang Guolu, Cheng Zaiquan, Tang Kaixue, Huang Xingqi,