کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4569695 | 1331351 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of S-alleles in pear (Pyrus communis L.) cv. 'Rocha' and other European cultivars
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
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چکیده انگلیسی
In this work we report the cloning and identification of S-RNase alleles responsible for gametophytic self-incompatibility (GSI) of 'Rocha' pear and of 13 other European pear cultivars that might be used as its pollinators. Partial sequences of S-RNase alleles were amplified by PCR with specific primers hybridising in conserved regions of previously identified S-RNase alleles of Pyrus communis, cloned and sequenced and the S-genotype of eight pear cultivars was fully determined. Three cultivars ('General Léclerc' (SqSl), 'Tosca' (SbSl) and 'Alexandrine Douillard' (SbSk)) shared no S-alleles with 'Rocha' (SaSe) and shall be totally compatible with this cultivar. None of the cultivars analysed showed an identical amplification pattern to the one observed in 'Rocha', so the other cultivars shall be at least semi-compatible. One new allele was identified in P. communis cv. 'Beurré d'Avril' (designated as St). The determination of both S-RNase alleles of cvs 'Rocha', 'Beurré Precoce Morettini' (SeSk) and 'Tosca' and the identification of one S-RNase allele in cvs 'Carapinheira' (Sb), 'Amêndoa' (Se), 'Pérola' (Sk) and 'Beurré d'Avril' (St) are important contributions for the effort recently developed worldwide to establish groups of sexual compatibility among European pears.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 113, Issue 1, 5 June 2007, Pages 13-19
Journal: Scientia Horticulturae - Volume 113, Issue 1, 5 June 2007, Pages 13-19
نویسندگان
Mariana Mota, Lucélia Tavares, Cristina M. Oliveira,