کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4569963 | 1331364 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Micropropagation of Chamomilla recutita (L.) Rauschert: A shock treatment model with growth regulators
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A protocol for in vitro clonal propagation of a Brazilian variety of chamomile (Chamomilla recutita (L.) Rauschert) is described. Nodal segments were submersed in liquid MS basal medium containing 1.0-4.0 mg lâ1 GA3 or thidiazuron (TDZ) or 0.5-4.0 mg lâ1 2,4-D for 2 h. After that, they were transferred to solid MS basal media without growth regulators and maintained for 2 months. This process avoided the callus development which was undesirable for shoot multiplication. The best treatment for shoot proliferation and plant rooting contained 1.0-2.0 mg lâ1 gibberellin (GA3) or 0.5 mg lâ1 2,4-D. Plants treated with 0.5 mg lâ1 2,4-D were subcultured for half-strength nitrogen solid MS medium and they were acclimatized two months later. The hardening process in the greenhouse was difficult and time consuming, however, 54% of plants survived and were successfully adapted to field conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 109, Issue 2, 29 June 2006, Pages 160-164
Journal: Scientia Horticulturae - Volume 109, Issue 2, 29 June 2006, Pages 160-164
نویسندگان
Alice Sato, Sharon S. de Lima, Vanessa R. Affonso, Maria Apparecida Esquibel, Celso L.S. Lage,