Polymorphisms of amplified mitochondrial DNA non-coding regions in Diospyros spp.

Universal primers were used to amplify mtDNA non-coding regions in Diospyros spp. including 6 related species and 20 genotypes of Diospyros kaki Thunb. The results showed: (1) 32 universal primers successfully amplified either introns or intergenic regions in Diospyros spp. A total of 119 bands were obtained, in which 110 were polymorphic. (2) Twenty three universal primers were used to analysis genetic diversity at the level of intra-specific, which revealed that the mitochondrial genomes had abundant variation during recombination. Chinese, Japanese PCNA genotypes were separated distinctly from each other by clustering analysis. (3) Two Chinese PCNA genotypes of Japanese persimmon, ‘Baogaitianshi’ and ‘Eshi No.1’, have unique bands to other materials, which showed they would have derived from the same female parent according to the maternal inheritance of mitochondrial genome.