کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4752297 1416121 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Past matrix stiffness primes epithelial cells and regulates their future collective migration through a mechanical memory
ترجمه فارسی عنوان
سفتی ماتریکس گذشته نخستین سلول های اپیتلیال را تنظیم می کند و مهاجرت جمعی آینده خود را از طریق یک حافظه مکانیکی تنظیم می کند
کلمات کلیدی
مهاجرت سلولی جمعی، مکانیک انتقال، ماتریکس غیر سلولی، سفتی، سختی، حافظه مکانیکی،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

During morphogenesis and cancer metastasis, grouped cells migrate through tissues of dissimilar stiffness. Although the influence of matrix stiffness on cellular mechanosensitivity and motility are well-recognized, it remains unknown whether these matrix-dependent cellular features persist after cells move to a new microenvironment. Here, we interrogate whether priming of epithelial cells by a given matrix stiffness influences their future collective migration on a different matrix - a property we refer to as the 'mechanical memory' of migratory cells. To prime cells on a defined matrix and track their collective migration onto an adjoining secondary matrix of dissimilar stiffness, we develop a modular polyacrylamide substrate through step-by-step polymerization of different PA compositions. We report that epithelial cells primed on a stiff matrix migrate faster, display higher actomyosin expression, form larger focal adhesions, and retain nuclear YAP even after arriving onto a soft secondary matrix, as compared to their control behavior on a homogeneously soft matrix. Priming on a soft ECM causes a reverse effect. The depletion of YAP dramatically reduces this memory-dependent migration. Our results present a previously unidentified regulation of mechanosensitive collective cell migration by past matrix stiffness, in which mechanical memory depends on YAP activity.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 146, November 2017, Pages 146-155
نویسندگان
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