کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4764099 | 1423377 | 2017 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent](/preview/png/4764099.png)
چکیده انگلیسی
Cross-linked enzyme aggregates (CLEAs) of nitrile hydratase (NHase) ES-NHT-118 from Escherichia coli were prepared by using ammonium sulfate as precipitating agent followed by cross-linking with dextran polyaldehyde for the first time. In this process, egg white was added as protein feeder for facilitating the formation of CLEAs. The optimal conditions of the immobilization process were determined. Michaelis constants (Km) of free NHase and NHase CLEAs were also determined. The NHase CLEAs exhibited increased stability at varied pH and temperature conditions compared to its free counterpart. When exposed to high concentrations of acrylamide, NHase CLEAs also exhibited effective catalytic activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Chemical Engineering - Volume 25, Issue 4, April 2017, Pages 487-492
Journal: Chinese Journal of Chemical Engineering - Volume 25, Issue 4, April 2017, Pages 487-492
نویسندگان
Liya Zhou, Haixia Mou, Jing Gao, Li Ma, Ying He, Yanjun Jiang,