کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4766091 1423896 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel fluorescent probe based on β-C-glycoside for quantification of bovine serum albumin
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A novel fluorescent probe based on β-C-glycoside for quantification of bovine serum albumin
چکیده انگلیسی
Two novel C-glycoside-coumarin fluorescent dyes are reported and their UV/Vis, fluorescence spectra and fluorescence-response to bovine serum albumin (BSA) are investigated and discussed. The fluorescence emission is red-shifted and quenched with the increase in polarity of solvents. Moreover, the fluorescence intensity of the dyes increased upon the addition of BSA in pH 7.4 phosphate buffer solution. An excellent linear relationship (R2 = 0.99495) for compound 1 was obtained between fluorescence intensity and bovine serum albumin concentration. The method show a good selectivity and sensitivity for BSA over few proteins, other ions and amino acids, which was suggested as high selective and sensitive fluorescent reagents for quantification of BSA in biological science.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 139, April 2017, Pages 334-343
نویسندگان
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