کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4926241 | 1363174 | 2017 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Utilization of by-product glycerol from bio-diesel plants as feedstock for 2,3-butanediol accumulation and biosynthesis genes response of Klebsiella variicola SW3
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Utilization of by-product glycerol from bio-diesel plants as feedstock for 2,3-butanediol accumulation and biosynthesis genes response of Klebsiella variicola SW3 Utilization of by-product glycerol from bio-diesel plants as feedstock for 2,3-butanediol accumulation and biosynthesis genes response of Klebsiella variicola SW3](/preview/png/4926241.png)
چکیده انگلیسی
The booming of biodiesel industry all over the world has led to generate a large amount (10% v/v) of crude glycerol, created an oversupply problem. Herein, we compared the product concentrations of major metabolic products attained from pure and crude glycerol biotransformation process using an adapted mutant strain Klebsiella variicola SW3. Real-time qPCR and glycerol dehydrogenase (GDH) enzyme activity assay revealed that the overexpression of GDH gene resulted in an increased GDH enzyme activity, led to a markedly boosted 2,3-butanediol (2,3-BD) production. Based on these results, the SW3 strain obtained from wild type strain Klebsiella variicola SRP3 displayed a 1.39-fold increased 2,3-BD production of 82.5Â g/L from 59.3Â g/L, yielding 0.62Â g/g using pure glycerol. However, in a batch culture, a final 33.5Â g/L of 2,3-BD was accumulated within 96Â h from 50Â g/L glycerol. Moreover, the strain SW3 withstanding high concentration (200Â g/L) of crude glycerol displayed 64.9 and 29.25Â g/L 2,3-BD in fed-batch and batch cultures respectively. Therefore, this bioconversion of crude glycerol to 2,3-BD -a value-added green product with potential industrial applications as a liquid fuel or fuel additive would represent a remarkable alternative to add value to the biodiesel production helping biodiesel industries development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Renewable Energy - Volume 114, Part B, December 2017, Pages 1272-1280
Journal: Renewable Energy - Volume 114, Part B, December 2017, Pages 1272-1280
نویسندگان
Md. Shafiqur Rahman, Chunbao (Charles) Xu, Kesen Ma, Haipeng Guo, Wensheng Qin,