کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4985048 1454501 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regular ArticleFabrication of cell-benign inverse opal hydrogels for three-dimensional cell culture
ترجمه فارسی عنوان
تولید منظم هیدروژل های اپال هیدروژنی سلول خونی برای کشت سلولی سه بعدی
کلمات کلیدی
ژلاتین، معکوس هیدروژل اپال، همکاری فرهنگی، داربست مکروپوروس، آلژینات،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی

Inverse opal hydrogels (IOHs) for cell culture were fabricated and optimized using calcium-crosslinked alginate microbeads as sacrificial template and gelatin as a matrix. In contrast to traditional three-dimensional (3D) scaffolds, the gelatin IOHs allowed the utilization of both the macropore surface and inner matrix for cell co-culture. In order to remove templates efficiently for the construction of 3D interconnected macropores and to maintain high cell viability during the template removal process using EDTA solution, various factors in fabrication, including alginate viscosity, alginate concentration, alginate microbeads size, crosslinking calcium concentration, and gelatin network density were investigated. Low viscosity alginate, lower crosslinking calcium ion concentration, and lower concentration of alginate and gelatin were found to obtain high viability of cells encapsulated in the gelatin matrix after removal of the alginate template by EDTA treatment by allowing rapid dissociation and diffusion of alginate polymers. Based on the optimized fabrication conditions, gelatin IOHs showed good potential as a cell co-culture system, applicable to tissue engineering and cancer research.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Colloid and Interface Science - Volume 494, 15 May 2017, Pages 389-396
نویسندگان
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