کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4989630 | 1456935 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a new bioaffinity stationary phase for lactose removal using a lactose-binding lectin immobilized onto polyaniline
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The global prevalence of individuals with lactose intolerance and galactosemia has created a new market for commercially available lactose-free food products. In this scenario, the use of systems containing lactose-binding molecules for lactose removal is quite promising. In this work, a new lectin from Brosimum gaudichaudii was purified, characterized and immobilized onto polyaniline aiming to develop a new system for lactose removal through bioaffinity chromatography. The lectin from Brosimum gaudichaudii (brosimin) was purified through sequential size exclusion chromatography, reaching a protein purification of 34-fold. Brosimin was characterized as a lactose-binding ion-independent lectin, with two subunits of 25Â kDa and 31Â kDa. The best support for brosimin immobilization was found as glutaraldehyde modified polyaniline, reaching 47% of lactose removal from skim bovine milk. Furthermore, considering the upscale process, the use of sequential reactors containing PANIG-brosimin could provide high levels of lactose removal, reaching a product with the requirements to be used by individuals with lactose and/or galactose intolerance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 185, 12 September 2017, Pages 54-60
Journal: Separation and Purification Technology - Volume 185, 12 September 2017, Pages 54-60
نویسندگان
K.A. Batista, C.N.S. Silva, P.M. Fernandes, I.T.N. Campos, K.F. Fernandes,