کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5008655 | 1462037 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design of amperometric urea biosensor based on self-assembled monolayer of cystamine/PAMAM-grafted MWCNT/Urease
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
In this work, we report novel amperometric urea biosensor based on immobilization of Urease enzyme onto self-assembled polyamidoamine grafted multiwalled carbon nanotube (MWCNT-PAMAM) dendrimers (from G1, first generation up to G5) on Au electrode. Bio-sensing electrode's performance parameters such as optimum pH, temperature, and applied potential together with reproducibility were investigated. The electrode based on fifth generation of MWCNT-PAMAM and urease enzyme showed excellent performance in urea detection at an applied potential of 0.45Â V with short response time of 3Â s, wide linear range of 1-20Â mM, detection limit of 0.4Â mM, and sensitivity of 6.6Â nA/mM. No significant response was detected in the presence of possible interferon molecules. Non-diluted human blood plasma has been used for real sample application where electrode showed excellent performance in urea detection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 254, January 2018, Pages 93-101
Journal: Sensors and Actuators B: Chemical - Volume 254, January 2018, Pages 93-101
نویسندگان
Muamer Dervisevic, Esma Dervisevic, Mehmet Åenel,