کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5008695 | 1462037 | 2018 | 6 صفحه PDF | دانلود رایگان |
- Multivalent glycosylated Cu:CdS QDs based bacterial detection and discrimination platform was developed.
- Glucose, stachyose and raffinose were used for construction bacterial detection and identification platform for the first time.
- Based on the response matrix from LDA, the method could identify 6 bacteria strains with an accuracy of 91.6%.
In this work, a rapid bacterial discrimination and detection system based on multivalent glycosylated Cu:CdS quantum dots (QDs) was presented. Cu:CdS QDs with excellent fluorescent properties were synthesized via a facile one-step method, then three natural carbohydrates, glucose, stachyose and raffinose, were coupled onto the surface of Cu:CdS QDs to construct glycosylated platforms. This is the first time that glucose, stachyose and raffinose were used for construction bacterial detection and identification platform. Bacterial discrimination was realized by analyzing the difference of binding strength between glycosylated Cu:CdS QDs and bacterial cell walls. Linear discriminant analysis was used to discriminate the response patterns, and six different bacterial samples could be identified within 30Â min. The greatest strengths of the bacterial discrimination platform based on multivalent glycosylated Cu:CdS QDs were rapid, simple and low-cost, there was no need for expensive reagents and instruments.
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Journal: Sensors and Actuators B: Chemical - Volume 254, January 2018, Pages 431-436