کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5008830 | 1462041 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High cell viability microfluidic electroporation in a curved channel
ترجمه فارسی عنوان
الکتروپارسینگ مایکروفیلیید زنده در سلول منحنی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this paper, by utilizing a curved channel, where the Dean flow is generated, the cell viability in the microfluidic electroporation chip has been significantly increased. The Dean vortex plays two major roles. Firstly, in the middle region of the curved channel, the cell solution is enveloped by the protective buffer, which keeps cells away from the metal electrodes throughout the electroporation process. Therefore, distasteful effects due to water electrolysis, such as air bubbles, Joule heating and pH changing, are mitigated or avoided. Secondly, in the ending region of the curved channel, the Dean vortex will recombine the split protective flow and neutralize positive and negative ions. Consequently, several hard-to-transfect cell types were successfully transferred with this device. Compared to conventional microfluidic electroporation devices, our electroporation chip can achieve flow-through electroporation with high viability and high transfection rate, while providing a continuous electroporation environment to address large doses of biological transfection requirement.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 250, October 2017, Pages 703-711
Journal: Sensors and Actuators B: Chemical - Volume 250, October 2017, Pages 703-711
نویسندگان
Dong Huang, Deyao Zhao, Jinhui Li, Yuting Wu, Wenbo Zhou, Wei Wang, Zicai Liang, Zhihong Li,