کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5008878 1462040 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two-in-one polydopamine nanospheres for fluorescent determination of beta-amyloid oligomers and inhibition of beta-amyloid aggregation
ترجمه فارسی عنوان
نانوسپیرهای پلییدوپامین دو نفره برای تعیین فلورسنتهای الیگومرهای بتا آمیلوئید و مهار تجمع بتا آمیلوئید
کلمات کلیدی
الیگومرهای بتا آمیلوئید، نانوسپیرهای پولیدپامین، بیماری آلزایمر، مهار کننده ها، فلورسانس، آپتامر،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Beta-amyloid (Aβ) oligomers are the reliable molecular biomarkers and crucial targets for the diagnosis and therapy of Alzheimer's disease (AD). In this work, we reported on a fluorescent platform for the selective detection of Aβ oligomers with polydopamine nanospheres (PDANS) as the nanoquencher. The carboxyfluorescein (FAM)-labeled DNA aptamers specific to Aβ oligomers showed poor fluorescence signal when absorbed onto the surface of PDANS. The specific binding of Aβ oligomer to the FAM-DNA caused the conformation change of the aptamer into a hairpin structure, thus inducing the release of FAM-DNA from the PDANS surface and making the fluorophore far away from the nanoquencher. Aβ oligomers were thus determined based on the increase in the fluorescence signal. The “signal on” fluorescent platform could be used for sensing of Aβ oligomers, but not for the monomers and fibrils. The detection limit was found to be 12.5 nM. More intriguingly, we found that PDANS efficiently prevented the aggregation of Aβ monomers. We believe that the two-in-one PDANS as both the nanoquencher and the inhibitor would found many applications for determination of Aβ oligomers in bodily fluids and drug-therapy of AD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 251, November 2017, Pages 359-365
نویسندگان
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