A rational design of ratiometric fluorescent probes based on new ICT/FRET platform and imaging of endogenous sulfite in living cells

- A new ICT/FRET platform was designed for ratiometric fluorescent probes.
- The probe has excellent selectivity and anti-interference for sulfite.
- The probe can detect endogenous sulfite in HepG2 and L-02 cells.
- The probe can preferably target mitochondria.

A new ICT/FRET platform composed of coumarin-hemicyanine dyad was rationally designed for ratiometric fluorescent probes. The platform could concert the spectral overlap and the emission shifts by changing ICT donor. The mitochondria-targeting probe was developed to sense endogenous sulfite in living cells with high selectivity, sensitivity and fast response.

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