کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5008954 1462038 2017 37 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a paper-based electrochemical immunosensor using an antibody-single walled carbon nanotubes bio-conjugate modified electrode for label-free detection of foodborne pathogens
ترجمه فارسی عنوان
توسعه یک ایمونسنسور الکتروشیمیایی کاغذی با استفاده از نانولوله های کربنی با یک تکه های تک تیرهای الکترودهای اصلاح شده زیستی کانژوگه برای تشخیص بدون برچسب از پاتوژن های غذا
کلمات کلیدی
بیوشیمیایی نانولوله کربنی آنتی بادی، استافیلوکوک اورئوس، بستر کاغذ، ایمونوسانسور الکتروشیمیایی، تشخیص بدون برچسب
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The need for low-cost, sensitive, and reliable sensors for the detection of whole bacterial cells in food samples without pre-treatment has been increasing. Outbreaks of foodborne diseases can be severe, especially in developing countries; however, most bio-detection tools are unaffordable. Here, we have developed a rapid and low-cost paper-based electrochemical immunosensor for label-free detection of Staphylococcus aureus, using antibody (Ab)-single walled carbon nanotube (SWCNT) bio-conjugates. Anti-S. aureus antibodies were covalently attached onto the SWCNTs, using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide coupling reagent. These Ab-SWCNT bio-conjugates were then immobilized on the working electrode, and the presence of S. aureus was detected by analyzing the change in peak current following antigen-antibody complex formation. Differential pulse voltammetry was performed with a bacterial concentration ranging from 10 to 107 colony forming units (CFU) mL−1. A selectivity assay using Escherichia coli B, Bacillus subtilis, and S. epidermidis (to examine cross-reactivity) showed that the sensor was specific to S. aureus. Moreover, this immunosensor showed a good linear relationship (R2 = 0.976) between the increase in peak current and logarithmic S. aureus concentration, with a rapid detection time (30 min) and a limit of detection of 13 CFU mL−1 in spiked milk samples. This low-cost immunosensor can be used for rapid detection of pathogens in actual food samples with high sensitivity and specificity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 253, December 2017, Pages 115-123
نویسندگان
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