کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5009776 | 1462045 | 2017 | 45 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly sensitive porous carbon and metal/carbon conducting nanofiber based enzymatic biosensors for triglyceride detection
ترجمه فارسی عنوان
بیوسنسورهای آنزیمی برای تشخیص تری گلیسرید بسیار جذاب کربن متخلخل و فلز / کربن نانوسیم
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Electrospinning was employed to synthesize a Ag nanoparticle (NP)-impregnated partially aligned and free-standing carbon nanofibers (CNFs) mat using a polyacrylonitrile (PAN) and silver nitrate blend followed by carbonization. Pyrolyzation of the PAN/AgNO3 blend produced the nanoporous CNFs, and the Ag NPs were grown within the CNFs via thermal decomposition of AgNO3. The fiber diameters of the synthesized CNFs ranged from 130 to 190 nm, and the size of the impregnated Ag NPs was â¼30 nm. The presence of the Ag NPs enhanced the electrical conductivity and promoted graphitization of the CNFs via the templating effect of the Ag NPs. These synthesized CNFs and AgCNFs nanocomposite were electrophoretically deposited onto indium tin oxide electrodes for detection of triglyceride molecules. Oxygen plasma treatment of the CNFs and AgCNFs surfaces resulted in enhanced loading of lipase and glycerol dehydrogenase bienzymes. The AgCNFs nanocomposite exhibited faster electron transfer than the CNFs, as corroborated by electrochemical impedance spectroscopy and cyclic voltammetry studies. Covalent functionalization via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide coupling chemistry on a nanoporous CNFs surface led to higher stability of the fabricated biosensor toward triglyceride detection. The sensitivity was four-fold higher for the AgCNFs (1.232 μA mg/dLâ1 cmâ2) bioelectrode compared with the CNFs (0.33 μA mg/dLâ1 cmâ2) over a wide detection range (25-500 mg/dL). These biosensors exhibited excellent selectivity, good reproducibility, and faster response (10 s). Thus, enhanced graphitization and electrical conductivity of nanoporous CNFs via incorporation of Ag NPs yields a promising platform for the detection of triglyceride (TG) biomolecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 246, July 2017, Pages 202-214
Journal: Sensors and Actuators B: Chemical - Volume 246, July 2017, Pages 202-214
نویسندگان
Kunal Mondal, Md. Azahar Ali, Chandan Singh, Gajjala Sumana, Bansi D. Malhotra, Ashutosh Sharma,