Miniaturized electrophoresis electrochemical protein sensor (MEEPS) for multiplexed protein detections

- Multiplex protein detection based on electrophoresis and electrochemical sensor was developed.
- Only 10 μL of sample was required for the system.
- Detection of 4 cytokines was demonstrated from single run.

We developed here a Miniaturized Electrophoresis Electrochemical Protein Sensor (MEEPS) platform that is capable of detecting specific and multiplexed signals from small volume of sample (10 μL) as well as reducing the effect of background interferences. The multiplexed capability and the reduced-effect of background interference are achieved using the electrophoresis unit of the MEEPS. The small foot-print system and sensitive signal detection are enabled via electrochemical sensors unit. Using cytokines as sample, results obtained from MEEPS reveal dynamic range of detection of representative cytokine, TNF-α, at 10 ng/mL-20 μg/mL and demonstrate multiplexed detections of four different cytokines IL-6, IFN-γ, TNF-α, and IL-8 from only 10 μL of sample.