Advanced 3D Spheroid Culture for Evaluation of Photodynamic Therapy in Microfluidic System

In this paper we present A549 (carcinoma) and MRC-5 (non-malignant) long-term (10 days) spheroids culture in a microfluidic system. Moreover, the evaluation of photodynamic therapy (PDT) procedure with 5-aminolevulinic acid (ALA) on 3D A549 and MRC-5 spheroids culture was performed. It was found that the spheroids are more resistant for ALA/PDT than monolayer culture. Photocytotoxic effect of the tested ALA concentration on A549 spheroids was noticed 8 days after PDT procedure. Selective effect of PDT procedure on carcinoma cells was observed.