کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5032533 | 1369985 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Valve interstitial cell shape modulates cell contractility independent of cell phenotype
ترجمه فارسی عنوان
شکل سلول درون سلولی دریچه سلول انقباضیه مستقل از فنوتیپ سلولی است
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
شکل سلولی، معماری سلولی، استرس تناسلی، فنوتیپ سلولی، سلول های داخل سلولی دریچه ای،
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
چکیده انگلیسی
Valve interstitial cells are dispersed throughout the heart valve and play an important role in maintaining its integrity, function, and phenotype. While prior studies have detailed the role of external mechanical and biological factors in the function of the interstitial cell, the role of cell shape in regulating contractile function, in the context of normal and diseased phenotypes, is not well understood. Thus, the aim of this study was to elucidate the link between cell shape, phenotype, and acute functional contractile output. Valve interstitial cell monolayers with defined cellular shapes were engineered via constraining cells to micropatterned protein lines (10, 20, 40, 60 or 80 µm wide). Samples were cultured in either normal or osteogenic medium. Cellular shape and architecture were quantified via fluorescent imaging techniques. Cellular contractility was quantified using a valve thin film assay and phenotype analyzed via western blotting, zymography, and qRT-PCR. In all pattern widths, cells were highly aligned, with maximum cell and nuclear elongation occurring for the 10 μm pattern width. Cellular contractility was highest for the most elongated cells, but was also increased in cells on the widest pattern (80 μm) that also had increased CX43 expression, suggesting a role for both elongated shape and increased cell-cell contact in regulating contractility. Cells cultured in osteogenic medium had greater expression of smooth muscle markers and correspondingly increased contractile stress responses. Cell phenotype did not significantly correlate with altered cell shape, suggesting that cellular shape plays a significant role in the regulation of valve contractile function independent of phenotype.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 49, Issue 14, 3 October 2016, Pages 3289-3297
Journal: Journal of Biomechanics - Volume 49, Issue 14, 3 October 2016, Pages 3289-3297
نویسندگان
Ishita Tandon, Atefeh Razavi, Prashanth Ravishankar, Addison Walker, Nasya M. Sturdivant, Ngoc Thien Lam, Jeffrey C. Wolchok, Kartik Balachandran,