Fabrication of electrochemical biosensor with vanadium pentoxide nano-interface for the detection of methylglyoxal in rice

- GSH as electrochemical biosensor's recognition element for methylglyoxal is reported.
- V2O5 nanoparticles were used to enhance electron transfer rate.
- The assay is based on the reaction of methylglyoxal with GSSG to form hemithioacetal.
- Detection limit of 2 nM with a swift response time of 18 s was achieved.
- Biosensor was used for the detection of methylglyoxal in rice with 100% recovery.

Increased consumption of raw and par-boiled rice results in the formation of methylglyoxal (MG) at higher concentration and leads to complications in diabetic patients. Highly sensitive electrochemical biosensor was developed using glutathione (GSH) as a co-factor with vanadium pentoxide (V2O5) as a nano-interface for MG detection in rice samples. The Pt/V2O5/GSH/Chitosan bioelectrode displayed two well-defined redox peaks in its cyclic voltammograms for MG reduction. This occurred as two electron transfer process where MG gained two electrons from oxidized glutathione disulfide and formed hemithioacetal. The current density response of the fabricated bioelectrode was linear towards MG in the concentration range of 0.1-100 μM with the correlation coefficient of 0.99, sensitivity of 1130.86 μA cm−2 μM−1, limit of detection of 2 nM and response time of less than 18 s. The developed bioelectrode was used for the detection of MG in raw and par-boiled rice samples.

254