کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5134377 | 1492222 | 2016 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Comparative proteomic analysis of serum of mice infected with Plasmodium berghei ANKA strain using two dimensional gel electrophoresis and matrix-assisted laser desorption ionization - time-of-flight mass spectrometry Comparative proteomic analysis of serum of mice infected with Plasmodium berghei ANKA strain using two dimensional gel electrophoresis and matrix-assisted laser desorption ionization - time-of-flight mass spectrometry](/preview/png/5134377.png)
- Proteomic analysis of serum of mice infected with Plasmodium berghei.
- 2D and MALDI analyses show differentially expressed proteins.
- Analysis leads to biomarker-based diagnostic tool.
Malaria, worldwide, affects around 500 million people with 2-3 million clinical cases every year. In this study, a proteomic approach was used to analyze the alterations in mice serum proteome induced by P. berghei infection; and identified number of differentially expressed proteins compared to control. Serum proteins were separated by two-dimensional electrophoresis (2DE). These proteins were further identified by matrix-assisted laser desorption or ionization - time-of-flight (MALDI-TOF) spectrometry. Important proteins identified include Serum amyloid A4 protein, Kelch-like protein, Glutathione-S-transferase Mu1. The identified proteins could have a significant role in developing a biomarker-based diagnostic tool for malaria.
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Journal: International Journal of Mass Spectrometry - Volume 410, 20 November 2016, Pages 57-62