Multiway calibration strategy with chromatographic data exploiting the second-order advantage for quantitation of three antidiabetic and three antihypertensive drugs in serum samples

The performance of both algorithms was compared in terms of predictive ability, showing relative error of prediction values below 10% in all cases. LOD values calculated are below 30 ng mL− 1 for all the studied drugs, which allow detection in human serum in patients under treatment. U-PLS/RBL has higher sensitivity and better detection and quantification limits for all the studied analytes; however results obtained by MCR-ALS enable its usage as well. Both methods provide comparable results for glibenclamide, glimepiride and gliclazide. With this multiway calibration strategy, the presence of enalapril, amlodipine and atenolol could be quantitated with high accuracy. Run time was reduced by 50% considering previous reports, as well as reduction of solvents, in accordance with green chemistry principles.