کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5139023 | 1494861 | 2017 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High throughput determination of ammonium and primary amine compounds in environmental and food samples
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this paper, an improved spectrofluorimetric method for the simultaneous and direct determination of ammonium and primary amine compounds is presented. The method is based on the derivatization of the analytes with o-phthaldialdehyde (OPA)/N-acetylcysteine (NAC) reagent using high throughput microplates, and OPA/NAC ratio has been optimized in order to suppress interference of ammonium on primary amine determination. Direct measurement of these two parameters is therefore possible with a global procedure time that does not exceed 10 min. Excellent limits of detection of 1.32 μM and 0.55 μM have been achieved for ammonium and primary amines, respectively. Reagent stability issues have also been addressed and formulation of reagents solution is described for improved reagents shelf life. The proposed protocol was finally applied and validated on real samples such as wine samples, compost extracts and wastewater.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 133, July 2017, Pages 216-221
Journal: Microchemical Journal - Volume 133, July 2017, Pages 216-221
نویسندگان
Fabien Robert-Peillard, Edwin Palacio Barco, Marco Ciulu, Carine Demelas, Frédéric Théraulaz, Jean-Luc Boudenne, Bruno Coulomb,