Discovery of novel double pyrazole Schiff base derivatives as anti-tobacco mosaic virus (TMV) agents

Two series of new double pyrazole Schiff base derivatives 4a-4t and 5a-5p as anti-TMV agents were designed and synthesized, in which compound 4j showed the most potent anti-TMV activity. Molecular docking revealed 5-chloro-3-methyl-1H-pyrazole as scaffold of compound 4j could insert into the nucleotide sequence (GAAGUU) of Ori-RNA target pocket stably.