کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5144962 | 1496894 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Voltammetric behavior of Mammeisin (MA) at a glassy carbon electrode and its interaction with Bovine Serum Albumin (BSA)
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The electrochemical oxidation of Mammeisin (MA) was studied in a solution containing acetone and 0.1 M phosphate buffer + 0.1 M KCl (pH = 5.3) at a glassy carbon electrode (GCE), using cyclic (CV) and square wave voltammetry (SWV). MA showed a quasi-reversible process, which is pH dependent and that involves the exchange of two electrons and two protons. The oxidation product was adsorbed by the electrode surface to form a film that blocks active sites over repetitive cyclic. Moreover, the interaction of MA and bovine serum albumin (BSA) was studied by CV and SWV at different pHs (5.4, 7.2, 9.5). As a result of the affinity binding with BSA, electrochemically inactive complex was formed. In addition, the oxidation potential of MA in the presence of BSA depends on the pH. The diffusion coefficients of both free and bound MA were estimated from the cyclic voltammetry data using the method developed by Randles-Sevich (Df = 9.85 Ã 10â 5 cm2 sâ 1 and Db = 1.27 Ã 10â 9 cm2 sâ 1) and the binding constant of MA-BSA complex, K = 3.47 Ã 102 L molâ 1, was obtained.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 119, February 2018, Pages 20-25
Journal: Bioelectrochemistry - Volume 119, February 2018, Pages 20-25
نویسندگان
Jules-Blaise Mabou Leuna, Sergeot Kungo Sop, Suzanne Makota, Evangeline Njanja, Thiery Christophe Ebelle, Anatole Guy Azebaze, Emmanuel Ngameni, Achille Nassi,