Enhanced oxygen reducing biocathode electroactivity by using sediment extract as inoculum

Autotrophic bacteria are able to catalyze cathodic oxygen reduction as a renewable and sustainable inexpensive catalyst. However, the performance of biocathode varied over reactors, and we still not know how inoculums affect this system. Using three different inoculum of wastewater (WW), sediment extract (SE) and soil extract (SO) in parallel reactors, we found that SE achieved the shortest setup time (17-25% shorter) as well as the highest power density compared to those of SO and WW. Cyclic voltammetry (CV) further revealed that the current densities of SE biocathodes (100 ± 1 A/m3) was 150% and 67% higher than those of WW biocathodes (40 ± 1 A/m3) and SO biocathodes (65 ± 1 A/m3). Community analysis showed the selective pressure on biocathode facilitated the growth of Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria families. Different from WW and SO biocathodes, Nitrospirae was selectively enriched in SE biocathodes, corresponding to an obvious increase in Unidentified Nitrospiraceae population at genus level, which may play an important role on the cathodic electroactivity. These results confirmed that sediment extract is a better bacteria source than soil and wastewater for the acclimation of autotrophic electroactive bacteria, and the community comparison provided broader knowledge on biocathode microbiology.