Magnetic nanoparticles with surfaces modified with chitosan-poly[N-benzyl-2-(methacryloxy)-N,N-dimethylethanaminium bromide] for lipase immobilization

- Superparamagnetic nanoparticles were surface-modified with a mixture of chitosan (CS) and poly [N-benzyl-2-(methacryloxy)-N,N-dimethylethanaminium bromide] (PQ) and characterized by FT-IR, XRD, SEM, TEM and TG analysis.
- The immobilization of lipase on the surface of prepared nanoparticles via EDC/sulfo-NHS activation have been done.
- High activity recovery was found (higher than 80%).
- The reusability of lipase-immobilized chitosan magnetic nanoparticles in catalytic cycles was tested and the residual activity was over 90% after 5th cycle and remained at a level 70-72.1% after 10th cycle.