Functionalization of PDMS modified and plasma activated two-component polyurethane coatings by surface attachment of enzymes

► The described technique allows the biofunctionalization of a two-component polyurethane (2C-PUR) coating. ► Creation of functional groups on the surface by migrating PDMS and plasma treatment. ► 2C-PUR systems with PDMS displayed a higher stability over a time period of at least 28 h. ► Chemical immobilization (chemisorption) of the enzyme to the surface showed statistically significant higher biological activity.