GlyHisGlyHis immobilization on silicon surface for copper detection

► Covalent immobilization of GlyHisGlyHis peptide on SiH surface using multistep strategy. ► ATR-FTIR and AFM characterization of the surface at each stage of the functionalization. ► Electrochemical detection of copper using the peptide-modified electrode. ► The recorded cyclic voltammograms showed a surface reversible process. ► Potential role of peptide-modified silicon in developing strategies for metals detection.