Detailed evaluation of protein adsorption on alumina surface through surface plasmon resonance phenomenon

► Alumina films were studied in terms of their initial protein adsorption through the SPR to investigate the adsorption of HSA. ► Na2HPO4·12H2O, NaH2PO4·2H2O and deionized water were mixed to coordinate the PB. ► The observed SPR angle behavior indicated that HSA was immediately adsorbed on alumina films. ► The adsorbed HSA was desorbed by the etching of the alumina layer. ► Thus, HSA cannot remain adsorbed on an alumina layer for a long time.