Topographic characterization of the self-assembled nanostructures of chitosan on mica surface by atomic force microscopy

- Nanocomposites of chitosan film were prepared by simple self-assembly from solvent media.
- Chitosan molecules assembled on mica surface of nanoparticles, fibril and membrane with varied chitosan concentration.
- Chitosan molecules assembled with different nanostructure under varied pH.
- The optimum drying temperature for forming chitosan membrane is about 65 °C.