Effect of two-step functionalization of Ti by chemical processes on protein adsorption

► The chemical treatment of a Ti surface resulted in significant changes in morphology. ► Ti(H2O2 + H3PO4) treatment led to the incorporation of phosphates into the oxide film. ► Calcium phosphate coatings can be uniformly grown on a chemically treated Ti surface. ► Ti(H2O2 + H3PO4) surface adsorb more albumin than does the Ti(NaOH) or reference Ti.