Chemical functionalization of surfaces for building three-dimensional engineered biosensors

► Nanostructured bienzymatic systems were designed with 3D architectures based on chemically functionalized supports and “biotin-avidin” affinity. ► The systems were built and assayed using bienzymatic nanostructures, glucose oxidase (GOx) and horseradish peroxidase (HRP). ► The bi-enzyme arrangement demonstrated biochemical activity by injecting the glucose substrate. ► The bienzyme system behaved effectively as biosensor for glucose using real samples of beverages. ► Nanotechnology using 3D-bioarchiteture is a promising tool for diabetic patients monitoring the quality of food/beverage.